In our laboratory a multidisciplinary approach combining Biochemistry, Microbiology, Molecular Biology, Protein Engineering, and Structural Biology has been followed to:

(i) advance the understanding of the catalytic, stability and structural fingerprints of bacterial oxidoreductive enzymes potentially involved in xenobiotics degradation and lignocellulose conversion, (ii) improve their performance and robustness using protein engineering approaches, including directed evolution, (iii) identify intermediates and products of enzymatic reactions through complementary information derived from Nuclear Magnetic Resonance (NMR) and Mass Spectrometry, (iv) use the acquired knowledge and tools to the set-up of multi(-step)-enzymatic or chemo-enzymatic green processes for the production of added-value compounds.

 

 
the biocatalyst cycle adapted from Schmidt et al. 2001, Nature, 409: 258

 

MET | Microbial & Enzyme Technology 2013