Mendes, S, Banha, C, Madeira, J, Santos, D, Miranda, V, Manzanera, M Ventura, MR, van Berkel, W JH, Martins, LO. 2016. Characterization of a bacterial pyranose 2-oxidase from Arthrobacter siccitolerans J Mol Catalys. B, Enzymatic DOI 10.1016/j.molcatb.2016.11.005

Sousa, AC, Piedade, MFMM, Martins, LO and Robalo. 2016. Eco-friendly synthesis of indo dyes mediated by a bacterial laccase. Green Chemistry. 2016, 18, 6063 – 6070.

Pistone, L, Ottolina, G, De, S, Romero, AA, Martins, LO, Luque, R. 2016. Encapsulated laccases for the room-temperature oxidation of aromatics: towards synthetic low-molecular-weight lignins. ChemSusCem DOI:10.1002/cssc.201501427


Brissos, V, Ferreira, M, Grass, G and Martins, LO. 2015. Turning a hyperthermostable metallo-oxidase into a laccase by directed evolution. ACS Catalysis 5: 4932-4941

Mendes, S, Catarino, T, Silveira, C, Todorovic, S and Martins, LO. 2015. Catalytic mechanism of BsDyP an A-type dye-decolourising peroxidase: neither aspartate nor arginine is individually essential for peroxidase activity. Cat. Sci. Technol. 5: 5196-5207.

Todorovic, S, Hildebrandt, P, Martins, LO. 2015. Surface enhanced resonance Raman detection of a catalytic intermediate of DyP-type peroxidase. Phys. Chem. Chem. Phys. 17:11954.  

Mendes, S, Gabriel, A, Catarino, T, Silveira, C, Turner, DL, Todorovic, S, Martins, LO. 2015. An integrated view of redox and catalytic properties of B-type PpDyP from Pseudomonas putida MET94 and its distal variants Arch Biochem Biophys. 574:99-107  

Sousa, AC, Piedade, MFMM, Duarte, MT, Martins, LO, Robalo. 2015. A novel enzymatic route for the synthesis of carbazole frameworks using bacterial CotA laccase. Green Chemistry. 17,1429  

Martins, LO, Durão, P, Brissos, V, Lindley, PF. 2015. Laccases of prokaryotic origin – enzymes at the interface of protein science and protein technology. Cell Mol Life Sci. 72: 911-22  

Mendes, S, Robalo, MP, Martins, LO. 2015. Bacterial Enzymes and Multi-Enzymatic Systems for Cleaning-up Dyes from the Environment in “Microbial Degradation of Synthetic Dyes in Waste Waters”. Ed. S. N. Singh. Springer. (p. 27-55) 


Sousa AC, Oliveira MC, Martins LO and Robalo MP. 2014. Towards the rational biosynthesis of substituted phenazines and phenoxazinones by laccases. Green Chem. 16: 4127–4136

Lang, W, Sirisansaneeyakul, S, Martins, LO, Ngiwsara, L, Sakairi, N, Pathom-Aree, W, Okuyama, M, Mori, H, Kimura, A. 2014. Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1T strain from the Mariana Trench. J Environ Management. 132: 155-64

Brissos, V, Gonçalves, N, Melo, EP, Martins LO. 2014. Improving kinetic or thermodynamic stability of an azoreductase by directed evolution. PLoS One. 9: e87209

Santos, A, Mendes, S, Brissos, V, Martins, LO 2014. New dye decolourising peroxidases from Bacillus subtilis and Pseudomonas putida: towards biotechnological applications. Appl. Microbiol. Biotechnol. 98:2053-2065


Lígia O. Martins. Biocatálise na despolimerização e valorização da lenhina. Boletim de Biotecnologia, Série 2, Número 3, Abril de 2013

Gonçalves, AM, Mendes, S, De Sanctis, D, Martins, LO, Bento, I 2013. The crystal structure of Pseudomonas putida PpAzoR: the active site revisited. FEBSJ. DOI:10.1111/febs.12568

Lang, W, Sirisansaneeyakul, S, Ngiwsara, L, Mendes, S, Martins, LO, Okuyama, M, Kimura, A. 2013. Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR1911: towards dye decolorization using a packed-bed metal affinity reactor. Bioresource Technol. 150:298-306

Sousa, AC, Martins, LO, Robalo, MP 2013. Laccase-catalysed homocoupling of primary amines towards the biosynthesis of dyes. Adv. Synth. Catal. 355:2908-2917

Sezer, M, Santos, A, Kielb, P, Pinto, T, Martins, LO, Todorovic, S. 2013. Distinct structural and redox properties of the heme active site in bacterial dye decolorizing peroxidase-type peroxidases from two subfamilies: resonance Raman and electrochemical study. Biochemistry, 52: 3074-3084 


Sezer, M, Genebra, T, Mendes, S, Martins, LO, Todorovic, S. 2012. A DyP-type peroxidase at a bio-compatible surface: structural and mechanistic insights. Soft Matter. 8: 10314-10321.

Rosado, T, Bernardo, P, Koci, K, Coelho, AV, Robalo, MP, Martins, LO. 2012. Methyl syringate: an efficient phenolic mediator for bacterial and fungal laccases. Bioresour. Technol. 124:371-378

Brissos, V, Chen, Z, and Martins, LO. 2012. The kinetic role of carboxylate residues in the proximity of the trinuclear centre in the O2 reactivity of CotA-laccase. Dalton Trans. 41, 6247-6255

Fernandes, AT, Lopes,C, Martins, LO, Melo, EP. 2012. Unfolding pathway of CotA-laccase and the role of copper on the prevention of refolding through aggregation of the unfolded state. Biochem. Biophys. Res. Commun. 422:442-6.

Silva, CS, Damas, JM, Chen, Z, Brissos, V, Martins, LO, Soares, CM, Lindley, PF and Bento, I. 2012. The role of Asp116 in the reductive cleavage of dioxygen to water in CotA-laccase: assistance during the proton transfer mechanism. Acta Cryst. D68: 186-193

Silva, C.S., Durão, P., Fillat, A., Lindley, P.F., Martins, L.O. and I. Bento 2012. Crystal structure of the multicopper oxidase from the pathogenic bacterium Campylobacter jejuni CGUG11284: characterization of a metallo-oxidase. Metallomics 4: 37-47.



Mendes, S, Farinha, A, Ramos, CG, Leitão, JH, Viegas, CA and Martins, LO. 2011. Synergistic action of azoreductase and laccase leads to maximal decolourisation and detoxification of model dye-containing wastewaters. Bioresource Technol. 102: 9852-9859.

Correia, B, Chen, Z, Mendes, S, Martins, LO and Bento, I. 2011. Crystallization and preliminary X-ray diffraction analysis of the azoreductase PpazoR1 from Pseudomonas putida MET94. Acta Crystallographic section F Struct Biol Cryst Commun 67:121-3.

Mendes, S, Pereira, L, Batista, C and Martins, LO. 2011. Molecular determinants of azoreduction activity in the strain Pseudomonas putida MET94. Appl. Microbiol. Biotechnol. 92:393-405.

Fernandes, AT, Pereira, MM, Silva, CS, Lindley, PF, Bento, I, Melo, EP and Martins, LO. 2011. The removal of a disulfide bridge of CotA-laccase changes the μs-ms time-scale dynamics involved in copper binding. J. Biol. Inorg. Chem. 16:641-651.



Bento, I, Silva, CS, Chen, Z, Martins, LO, Lindley, PF and Soares, CM. 2010. Mechanisms underlying dioxygen reduction in laccases. Structural and modelling studies. BMC Structural Biology 10:28.

Fernandes, AT, Damas, J, Soares, CM, Todorovic, S, Huber, R, Pogni, R and Martins, LO. 2010. The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity. FEBS J 277:3176-3179

Chen, Z, Durão, P, Silva, CS, Pereira, MM, Todorovic, S, Hildebrandt, P, Bento, I, Lindley, PF and Martins, LO. 2010. The role of E498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis. Dalton Transactions 39:2875-2882



Pereira, L, Coelho, AV, Viegas, CA, Ganachaud, C, Iacazio, G, Tron, T, Robalo, MP and Martins, LO. 2009. On the mechanism of biotransformation of the anthraquinonic dye Acid Blue 62 by laccases. Adv Synth Catal 351:1857-1865

Brissos, V, Pereira, L, Munteanu, F-D, Cavaco-Paulo, A and Martins, LO. 2009. Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high redox potential dyes Biotechnol J 4:558-63

Pereira, L, Coelho, AV, Viegas, CA, Correia dos Santos, MM, Robalo, MP and Martins, LO. 2009. Enzymatic biotransformation of the azo dye Sudan Orange G with bacterial CotA-laccase. J Biotechnol 139:68-77

Fernandes, AT, Martins, LO and Melo, EP. 2009. The hyperthermophilic nature of the metallo-oxidase from Aquifex aeolicus. Biochim Biophys Acta-Proteins and Proteomics 1794:75-83



Durão, P, Chen, Z, Silva, CS, Soares, CM, Pereira, MM, Todorovic, S, Hildebrandt, P, Bento, I, Lindley, PF and Martins, LO 2008 Proximal mutations at the T1 copper site of CotA-laccase spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants. Biochem J 412:339-46

Durão, P, Chen, Z, Fernandes, AT, Hildebrandt, P, Murgida, DH, Todorovic, S, Pereira, MM, Melo, EP and Martins, LO. 2008. Copper incorporation into recombinant CotA-laccase   from Bacillus subtilis - Characterization of fully Cu-loaded enzymes. J Biol Inorg Chem 13:183-93



Melo, EP, Fernandes, AT, Durão, P, Martins, LO. 2007. Insight into stability of CotA-laccase from the spore coat of Bacillus subtilis. Biochem Soc Trans 35: 1579-82

Sánchez-Sutil, MC, Gómez-Santos, N, Moraleda-Muñoz, A, Martins, LO, Pérez, J and Muñoz-Dorado, J. 2007. Differential expression of the three multicopper oxidases from Myxococcus xanthus. J Bacteriol 189:4887-98

Fernandes, AT, Soares, CM, Pereira, MM, Huber, R, Melo, EP and Martins, LO. 2007 A robust metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus. FEBS J 274:2683-94


before 2006

Durão, P, Bento, I, Fernandes, AT, Melo, EP, Lindley, PF and Martins, LO. 2006. Perturbations of the T1 copper site in the CotA-laccase from Bacillus subtilis: structural, biochemical, enzymatic and stability studies. J Biol Inorg Chem 11:514-26.

Bento, I, Martins, LO, Lopes, GG, Carrondo, MA and Lindley, PF. 2005. Dioxygen reduction by the multicopper oxidases: a structural perspective. Dalton Transactions. 3507-13.

Enguita, FJ, Marçal,D,Martins, LO, Lindley, PF and Carrondo, MA. 2004. Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis. J Biol Chem 279:23472-76.

Enguita, FJ, Martins, LO, Henriques, AO and Carrondo, MA. 2003. Crystal structure of a bacterial endospore coat component: a laccase with enhanced thermostability properties. J Biol Chem 278: 19416-25.

Enguita, FJ, Matias, PM, Martins, LO, Plácido, D, Henriques, AO and Carrondo, MA. 2002. Spore-coat laccase CotA from Bacillus subtilis: Crystallization and preliminary X-ray characterization by the MAD method. Acta Crystallographica, 58:1490-93.

Martins, LO, Soares, CM, Pereira, MM, Teixeira, M, Costa, T, Jones, GH and Henriques, AO. 2002. Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat. J Biol Chem 277: 18849-59.




MET | Microbial & Enzyme Technology 2013